The UN aid agency in Gaza says it has suspended all aid shipments, accusing the Hamas government of seizing hundreds of tonnes of food supplies.
Ten lorries carrying flour and rice were taken from the Palestinian side of the Kerem Shalom crossing, the UN's Relief and Works Agency (Unrwa) said.
Hamas admitted a "mistake" had been made and says it will return the goods.
But Unwra says deliveries will not restart until it has assurances that such seizures will not happen again.
Gaza is facing a humanitarian crisis after Israel's three-week offensive.
About half the population is dependent on UN food aid.
Israel intensified a blockade on the territory 19 months ago when Hamas took over the territory.
The lifting of the blockade is among Hamas' demands for agreeing a long-term truce with Israel.
On Friday the group's exiled leader, Khaled Meshaal, told a rally in Syria that Israel still had not given the necessary undertakings for such a truce.
Second incident
Unrwa said the food had been imported from Egypt, and had been due to be collected by its staff at the Kerem Shalom crossing in southern Gaza on Friday.
"The food was taken away by trucks contracted by the ministry of social affairs," the agency said in a statement.
It said aid deliveries would only be resumed if Hamas returned all the aid and provided "credible assurances" that it would not happen again.
It was the second incident in three days. On Tuesday, 3,500 blankets and more than 400 food parcels were seized at gunpoint from a distribution centre in Gaza, the UN said.
The Hamas government's social affairs minister, Ahmed al Kurd, ordered "the aid to be returned to the agency if it turns out it is indeed its property", Hamas spokesman Fawzi Barhum said, according to AFP news agency.
He said no Hamas or Unrwa representatives had been present at the crossing when drivers loaded up the aid supplies, and the drivers had assumed they belonged to the Hamas government.
Although the UN, as an organisation, does not negotiate with Hamas, its relief agency in Gaza has to have some contact with the faction for practical reasons, says the BBC's Aleem Maqbool in Gaza City.
UN-Hamas tensions
Earlier in the week, Mr Kurd warned Unrwa not to "become a political player in Gaza". He said all aid should be distributed through Hamas.
But Hamas's rapid attempt to rectify the situation - at least once Unrwa announced its suspension of imports publicly - suggests it does understand how crucial the UN's aid work is, our correspondent says.
Hamas itself has given very limited financial assistance to some of the thousands of Gazans whose homes were destroyed in the Israeli offensive.
The perception of many in the territory is that the group is only helping its own supporters, he adds.
The UN has increased its food distribution in recent weeks to cover 900,000 of Gaza's population of 1.5 million following Israel's offensive against Hamas that began in December.
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